EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.34 | aminopterin | the DHFR inhibitor aminopterin inhibits tetrahydrobiopterin recycling. Aminopterin has no effect alone but slightly reduced L-citrulline formation and cGMP accumulation in the presence of dihydrobiopterin | Homo sapiens | |
1.5.1.34 | aminopterin | the DHFR inhibitor aminopterin inhibits tetrahydrobiopterin recycling. Aminopterin has no effect alone but slightly reduced L-citrulline formation and cGMP accumulation in the presence of dihydrobiopterin | Sus scrofa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.34 | additional information | - |
additional information | Michaelis-Menten kinetics of dihydrofolate reductase-catalyzed (6R)-5,6,7,8-tetrahydro-L-biopterin recycling, the enzyme shows high affinity for dihydrobiopterin | Sus scrofa | |
1.5.1.34 | additional information | - |
additional information | MichaelisMenten kinetics of dihydrofolate reductase-catalyzed (6R)-5,6,7,8-tetrahydro-L-biopterin recycling | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.5.1.34 | cytosol | - |
Homo sapiens | 5829 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.34 | 6,7-dihydropteridine + NADPH + H+ | Homo sapiens | - |
5,6,7,8-tetrahydropteridine + NADP+ | - |
r | |
1.5.1.34 | 6,7-dihydropteridine + NADPH + H+ | Sus scrofa | - |
5,6,7,8-tetrahydropteridine + NADP+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.34 | Homo sapiens | P09417 | - |
- |
1.5.1.34 | Sus scrofa | Q8MJ30 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.34 | aorta | - |
Sus scrofa | - |
1.5.1.34 | endothelial cell | - |
Homo sapiens | - |
1.5.1.34 | endothelial cell | aortic | Sus scrofa | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.34 | 6,7-dihydropteridine + NADPH + H+ | - |
Homo sapiens | 5,6,7,8-tetrahydropteridine + NADP+ | - |
r | |
1.5.1.34 | 6,7-dihydropteridine + NADPH + H+ | - |
Sus scrofa | 5,6,7,8-tetrahydropteridine + NADP+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.34 | DHPR | - |
Homo sapiens |
1.5.1.34 | DHPR | - |
Sus scrofa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.34 | 37 | - |
assay at | Homo sapiens |
1.5.1.34 | 37 | - |
assay at | Sus scrofa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.34 | 7.4 | - |
assay at | Homo sapiens |
1.5.1.34 | 7.4 | - |
assay at | Sus scrofa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.34 | NADP+ | - |
Homo sapiens | |
1.5.1.34 | NADP+ | - |
Sus scrofa | |
1.5.1.34 | NADPH | - |
Homo sapiens | |
1.5.1.34 | NADPH | - |
Sus scrofa |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.5.1.34 | metabolism | inhibition of de novo biosynthesis of tetrahydrobiopterin by preincubating the cells for 24 h with the GTP cyclohydrolase inhibitor DAHP (10 mM) diminishes L-citrulline formation. Addition of dihydrobiopterin fully restores L-citrulline formation. In addition to its effect on arginine-to-citrulline conversion, pretreatment of the cells with DAHP reduces A23187-induced cGMP accumulation. Addition of tetrahydrobiopterin or dihydrobiopterin not only antagonizes the inhibitory effect of DAHP but also increases cGMP accumulation. (6R)-5,6,7,8-Tetrahydro-L-biopterin availability regulates nitric oxide and superoxide formation by endothelial nitric oxide synthase (eNOS). At low tetrahydrobiopterin or low tetrahydrobiopterin to 7,8-dihydrobiopterin ratios the enzyme becomes uncoupled and generates superoxide at the expense of NO | Sus scrofa |
1.5.1.34 | metabolism | inhibition of de novo biosynthesis of tetrahydrobiopterin by preincubating the cells for 24 h with the GTP cyclohydrolase inhibitor dihydroxyacetone phosphate (10 mM) diminishes L-citrulline formation. Addition of dihydrobiopterin fully restores L-citrulline formation. In addition to its effect on arginine-to-citrulline conversion, pretreatment of the cells with dihydroxyacetone phosphate reduces A23187-induced cGMP accumulation. Addition of tetrahydrobiopterin or dihydrobiopterin not only antagonizes the inhibitory effect of dihydroxyacetone phosphate but also increases cGMP accumulation. (6R)-5,6,7,8-Tetrahydro-L-biopterin availability regulates nitric oxide and superoxide formation by endothelial nitric oxide synthase (eNOS). At low tetrahydrobiopterin or low tetrahydrobiopterin to 7,8-dihydrobiopterin ratios the enzyme becomes uncoupled and generates superoxide at the expense of NO | Homo sapiens |
1.5.1.34 | physiological function | the enzyme catalyzes (6R)-5,6,7,8-tetrahydro-L-biopterin recycling in endothelial cytosols | Homo sapiens |
1.5.1.34 | physiological function | the enzyme catalyzes (6R)-5,6,7,8-tetrahydro-L-biopterin recycling in endothelial cytosols with high affinity for dihydrobiopterin | Sus scrofa |